The results of the qPCR primers will be analyzed using a specific online tool. Since this program is under a license, the first step of the quality control analysis will be performed as a demonstration. After the basic quality controls are performed, we will use another program to normalize the data and obtain the final results : the gene expression levels of the candidate genes in WT vs. mutants. More information regarding the steps to carry out when analyzing RT-qPCR data will be provided during the last afternoon of the plant biology practicals, on Nov. 22.
The plan for this afternoon is :
Further information will be provided during the TP session.